2024 XRET Program

6:00-9:00

BBQ Dinner (MBL Club)

Sponsors
Iwaki, Aquaneering, Tecniplast, Xenopus One and Xenopus Express

7:00-8:40

BREAKFAST

8:40-8:45

Introduction and Welcoming Remarks (Speck Auditorium)
Jennifer Landino, Coral Zhou, Helen Willsey

Session 1. Resources

8:45- 9:00

Nikko-Ideen Shaidani (Marine Biological Laboratory)
Xenopus Mutant Resource

9:00-9:15

Annie Godwin (University of Portsmouth
European Xenopus Resource Centre (EXRC) updates and priorities

9:15-9:30

Aaron Zorn (University of Cincinnati)
Xenbase: genome annotation improvements

9:30-9:45

Katie Stein (NIH)
NIH Funding and Resources for Xenopus Research

9:45-10:00

Jacques Robert (University of Rochester)
Xenopus Research Resource for Immunobiology (XRRI): new genetic tools and leading effort on biological impact of microplastic water contaminants

10:00-10:15

Gary Gorbsky (Oklahoma Medical Research Foundation)
Expanding the Toolset: Creation of Novel Xenopus laevis and Xenopus tropicalis Cell Lines and Their Applications in Gene Editing

10:15-10:30

Dominique Alfandari (University of Massachusetts, Amherst)
Producing novel monoclonal antibody to Xenopus, Axolotl and Mouse to improve rigor and reproducibility

10:30-10:45

Doug Houston (University of Iowa)
DSHB: Sharing monoclonal antibodies through open science

10:45-11:00

COFFEE BREAK

Session 2. Development I

11:00-11:15

Jakub (Kuba) Sedzinski (University of Copenhagen)
Phenotypic profiling of developing mucociliary epithelium

11:15-11:30

Rachel Miller (University of Texas Health, McGovern Medical School)
Advancing our understanding of kidney development and birth defects using innovative technologies

11:30-11:45

Casey Griffin (New York University)
Deciphering the mechanisms of Nager syndrome using Xenopus tropicalis

11:45-12:00

Andrea Wills (University of Washington)      
Deciphering the contributions of carbohydrate and nucleotide metabolism to Xenopus regeneration

12:00-1:45

LUNCH

Session 3. Reconstitution using extracts

1:45-2:00

Yasuhiro Arimura (Fred Hutch Cancer Center)
Applying cryo-EM to Xenopus egg extract system to elucidate the structural basis of biological events on chromosomes

2:00-2:15

Susannah Rankin (Oklahoma Medical Research Foundation)
Protein tricks in extracts and embryos

2:15-2:30

Jesse (Jay) Gatlin (University of Wyoming)
The Shape of Extract: Creating Discrete Volumes of Cell-Free Xenopus Extracts to Study Fundamental Cellular Processes

2:30-2:45

Gembu Maryu (Yang Lab, University of Michigan)
Visualization and manipulation of Cdk1 oscillations in water-in-oil droplets

2:45-3:00

Martin Wühr (Princeton University)
Protein turnover measurements in embryogenesis

3:00-3:30

COFFEE BREAK

Session 4. Biophysical and Mathematical Modeling

3:30-3:45

Sarah Woolner (University of Manchester)
Measuring and applying tension in Xenopus: adventures with Flipper-TR and Lego

3:45-4:00

Lance Davidson (University of Pittsburgh)
New and improved! Tools to explore extreme mechanics of morphogenesis

4:00-4:15

Kristian Franze (University of Cambridge)
Measuring and manipulating tissue mechanics to understand brain development in Xenopus laevis

4:15-4:30

Shinuo Weng (Johns Hopkins University)
TFlux and Flex: Building Insights into Cell and Tissue Mechanics with Useful Fluctuation

Session 5. Development II

4:30-4:45

Adrian Thompson (Brown University)
Novel approaches for modeling neurodevelopmental effects of sodium channel dysregulation in the developing brain of Xenopus laevis tadpoles

4:45-5:00

Richard Behringer (University of Texas, MD Anderson Cancer Center)
Role of anti-Müllerian hormone in Xenopus tropicalis

5:00-5:15

David Vijatovic (Sweeney lab, Institute of Science and Technology Austria)
Adeno-Associated Viral Tools to Trace Neural Development and Connectivity in Xenopus Frogs

5:15-5:30

Vanja Stankic (Chen Lab, University of Texas, MD Anderson Cancer Center)
Cell at a Time: Unveiling the Cellular Landscape of Xenopus Lungs During Metamorphosis

5:30-5:45

Pat Kearns (University of Massachusetts, Boston)
There’s what on my Xenopus? Manipulating the Xenopus laevis microbiome to understand immunological function and amphibian microbial ecology

Bruno Reversade (A*STAR)
Of Frogs & Men: modeling Mendelian disease to better understand disease pathogenesis

6:00-8:00

DINNER

KEYNOTE

8:00-9:00

Thomas Naert (University of Ghent)
Non-random DNA repair allows predictable genome engineering

9:00-11:00

MIXER @ Captain Kidd

Sunday October 13, 2024

7:00-8:45

BREAKFAST

Session 6. Omics

8:45-9:00

Leonid Peshkin (Harvard University)
XePA: Xenopus Protein Atlas

9:00-9:15

Jose Abreu (Harvard University)
Xenopus Embryo Atlas: every single cell?

9:15-9:30

Hui Chen (University of South Carolina)
Quantifying Nascent Transcription in Early Embryogenesis

9:30-9:45

Shinhyeok Chae (Ulsan National Institute of Science and Technology (UNIST)
Cross-species integration of Xenopus single-cell sequencing data

9:45-10:00

Nayeli Reyes-Nava (Wallingford lab, University of Texas at Austin)
Proteomics, AlphaFold, and disease modeling in Xenopus

10:00-10:15

Karel Dorey (University of Manchester)
Uncovering the mechanisms underpinning regenerative neurogenesis using single-cell transcriptomics

10:15-10:30

Can Aztekin (Swiss Federal Institute of Technology Lausanne)
Xenopus regeneration and new sequencing approaches

10:30-11:00

COFFEE BREAK

11:00-12:00

Junior Strategic Planning

12:00-1:30

LUNCH

1:30-2:30

Strategic planning (including relay of junior requests)

Session 7. Physiology and Disease Modeling

2:30-2:45

Shiri Kult Perry (Shubin lab, University of Chicago)
The Xenopus respiratory system reveals common tetrapod mechanisms for growth, regeneration, and healing.

2:45-3:00

Nicole Edwards (Zorn Lab, Cincinnati Children’s Hospital)
Discovering the developmental basis of endosome trafficking disorders and congenital anomalies using Xenopus

3:00-3:15

Lydia Youmans (University of Texas, Houston)
As the Frog Folds: Exploring Human Genetic Variants of Neural Tube Defects in Xenopus

3:15-3:30

Mustafa Khokha (Yale University)
Are ion channels morphogens? Surprising results inspired by patient based gene discovery.

3:30-3:45

Kris Vlemnickx (University of Ghent)
Modeling human cancer and inherited disease, expanding the experimental landscape to oncogenes and the non-coding genome

3:45-4:00

COFFEE BREAK

Continuation of Disease Modeling

4:00-4:15

Adrian Romero (Miller Lab, UTHealth Houston)
Frogs, Bone, and Kidney: Exploring Developmental Processes in Xenopus

4:15-4:30

Jack Govaerts (Schoborg Lab, University of Wyoming)
Building a 3D Developmental Atlas of X. laevis with Micro CT

4:30-4:45

Engin Deniz (Yale University)
Unveiling the complexities of CSF circulation using Xenopus and OCT imaging

4:45-5:00

Andrea Vu (Robert Lab, University of Rochester)
T cell gene expression profiling in Xenopus laevis during Mycobacteria infection

Session 8. Cell Biology

5:00-5:15

Saurabh Kulkarni (University of Virginia)
Understanding multiciliogenesis in Xenopus

5:15-5:30

Enzo Bresteau (Northwestern University)
Apical Size Reduction by Macropinocytosis Alleviates Tissue Crowding

5:30-5:45

Jaeho Yoon (National Cancer Institute)
Limitations of Existing Proximity Labeling Methods and the Development of a New Approach

5:45-6:00

Leslie Sepaniac (Bement Lab, University of Wisconsin)
Synthetic constitution of traveling Rho GTPase waves at the cell cortex

6:00-8:00

DINNER

KEYNOTE

8:00-9:00

Rebecca Heald (University of California, Berkeley)
Using Xenopus to investigate the effects of ploidy on cell and developmental biology

9:00-11:00

MIXER @ Captain Kidd

Monday, October 14, 2024

7:00-9:00

Breakfast

9:00-10:30

XRET GO Jamboree Workshop

10:30-12:00

Cryopreservation Workshop and NXR Tour

12:00

LUNCH AND DEPARTURE