Sample Course Schedule
CRISPR/Cas9 Genome Editing: A Hands-on Experience
Time |
Wednesday |
Thursday |
Friday |
Saturday |
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7:00 |
Breakfast 7 - 8:30 am |
Breakfast 7 - 8:00 am |
Breakfast 7 - 8:00 am |
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7:30 |
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8:00 |
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8:30 |
IT Session - Meigs: Access to network and shared drives |
Check embryos |
Check embryos |
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9:00 |
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Lab - Loeb 207: Injections of gRNAs and dyes, |
Lab - Loeb 207: Injections, Score Phenotypes, dechorinate, fix and do phalloidin and dapi stain |
Lab - Loeb 207: score second round of phenotypes, wrap up discussion about phenotypes and CRISPR, discussion on ethics and CRISPR. Archiv's tour and Gift shop time |
9:30 |
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10:00 |
Arrival & Check-In: 10:00 am - 12:00 pm |
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10:30 |
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11:00 |
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11:30 |
Tour - MRC tank room |
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12:00 |
Lunch Noon - 1:00 |
Lunch - Noon to 1:30 pm |
Lunch - Noon to 1:30 pm |
Lunch - Noon to 1:30 pm |
12:30 |
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1:00 |
MBL Orientation & Safety Training |
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1:30 |
Lab - Loeb 207: Intro to Imaging competition. Intro to fluorescent imaging, GFP Lines, Fluorescent Staining, maybe hair cell staining & Time Lapse Development |
Lab - Loeb 207: Image fixed, stained animals |
Departure & Check-Out: 1:30 pm |
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2:00 |
Lecture - Loeb 263: Introduction to embryology & development & overview of how CRISPR works |
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2:30 |
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3:00 |
Lab - Loeb 207: Introduction to Microscopes and learn how to inject |
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3:30 |
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4:00 |
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4:30 |
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5:00 |
Dinner 5:30 to 6:30 pm |
Pizza & Movie Night beginning at 6 pm, Meigs Room, Swope Center |
Dinner 5:30 to 6:30 pm |
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5:30 |
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6:00 |
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6:30 |
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Work on images for image competition, submit by 10pm |
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7:00 |
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7:30 |