Microscopes in Development
General Overview:
A custom-built line scanning confocal microscope with two opposing views that is capable of imaging samples up to several millimeters in length at high resolution and fast speed.
Commercial or Pre-commercial:
Pre-Commercial
Which Facility:
Imaging Innovation Initiative
Imaging Mode:
Optical
If Optical:
Confocal
Microscope Stand:
4-pi configuration custom designed
Features:
Capable of imaging large areas and volumes rapidly with high NA objective lenses.
Simultaneous two-sided illumination and detection to increase volumetric speed and obtain sample information that may be occluded from a single view.
Simultaneous two-sided illumination and detection to increase volumetric speed and obtain sample information that may be occluded from a single view.
Laser Wavelengths:
405 nm, 488 nm, 561 nm, 640 nm
Excitation/Emission Filters:
Quad band filter 405/488/561/640 lasers. A filter wheel with an individual band bass filter for each laser excitation wavelength.
Detector:
Camera
Imaging lenses:
25x, 1.1 NA Water Immersion
40x, 1.25 NA Silicone
60x, 1.4 NA Oil
100x, 1.35 NA Silicone
100x, 1.49 NA Oil
40x, 1.25 NA Silicone
60x, 1.4 NA Oil
100x, 1.35 NA Silicone
100x, 1.49 NA Oil
Microscope Control software:
Custom designed Graphical User Interface in Matlab
Image Analysis Software:
Custom designed in Matlab
CO2:
No
Humidity:
No
Light Tight:
Yes
Sample mounting requirements/constraints:
Convention sample mounting approaches. Glass Bottom Petridish
Location - Building and Room:
Lillie 219
Contact Person:
General Overview:
Home-built TIRF Microscope capable of illuminating samples in a ring-TIR mode or a single sided TIR.
Commercial or Pre-commercial:
Pre-Commercial
Which Facility:
Imaging Innovation Initiative
Imaging Mode:
Optical
If Optical:
Widefield/TIRF
Microscope Stand:
Inverted
Features:
Epi illumination or TIR illumination
Laser Wavelengths:
405 nm, 488 nm, 561 nm, 640 nm
Excitation/Emission Filters:
Quad band filter 405/488/561/640 lasers. Individual emission filters can be added manually.
Detector:
Camera
Imaging lenses:
100x, 1.49 NA TIRF lens
Microscope Control software:
Custom designed Graphical User Interface in Matlab and u-Manager
Image Analysis Software:
CO2:
No
Humidity:
No
Light Tight:
Yes
Sample mounting requirements/constraints:
Conventional sample mounting.
Location - Building and Room:
Lillie 219
Hourly Rate for Use:
$25.00
Contact Person:
General Overview:
A home built dual-view SPIM system using multi-immersion lenses for imaging clear brain tissues. This system is also suitable for imaging samples in water/sea water.
Commercial or Pre-commercial:
Pre-Commercial
Which Facility:
Imaging Innovation Initiative
Imaging Mode:
Optical
If Optical:
Lightsheet
Microscope Stand:
Custom designed frame
Features:
Suitable for imaging large samples at high volumetric speed.
Laser Wavelengths:
405 nm, 488 nm, 561 nm, 640 nm
Excitation/Emission Filters:
Quad band filter for 405/488/561/640 lasers.
Detector:
Camera
Imaging lenses:
14x, 0.4 NA Multi-immersion lenses
23x, 0.7 NA Multi-immersion lenses
23x, 0.7 NA Multi-immersion lenses
Microscope Control software:
Custom designed Graphical User Interface in Matlab
Image Analysis Software:
Fiji/Matlab
CO2:
No
Humidity:
No
Light Tight:
No
Sample mounting requirements/constraints:
Conventional sample mounting
Location - Building and Room:
Lillie 219
Contact Person:
General Overview:
This system facilitates illuminating samples using a linearly polarized light in Epi or TIRF mode and detects fluorescence emission in parallel and perpendicular directions using a single sCMOS camera. Anisotropy imaging can provide information about protein-protein interaction or protein clustering.
Commercial or Pre-commercial:
Pre-Commercial
Which Facility:
Imaging Innovation Initiative
Imaging Mode:
Optical
If Optical:
Widefield/TIRF
Microscope Stand:
Inverted
Features:
405 nm, 488 nm, 561 nm, 640 nm
Laser Wavelengths:
A single band pass filter appropriate for the illumination.
Detector:
Camera
Imaging lenses:
100x, 1.49 NA Oil TIRF lens
60x, 1.4 NA Oil lens
60x, 1.4 NA Oil lens
Microscope Control software:
Custom designed Graphical User Interface in Matlab
Image Analysis Software:
Custom designed Image Analysis Graphical User Interface in Matlab
CO2:
No
Humidity:
No
Light Tight:
Yes
Sample mounting requirements/constraints:
Conventional sample mounting
Location - Building and Room:
Lillie 219
Contact Person: