2024 SES Microbial Methods Reading List
There are no required textbooks in this course, but Brock Biology of Microorganisms , 12th ed. by Madigan, Martinko, Dunlap and Clark (2009) is highly recommended (ISBN: 0-132-32460-1)
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Source for Topic | Section | pp. | ||
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1: Introduction |
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Brock Biology of Microorganisms, 12th Ed. |
1.0-1.4 Introduction |
2 - 7 |
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1.9-1.10 Microbial diversity and modern era |
18 - 21 |
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2.5 Elements of cell and viral structure |
33 - 35 |
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2.6 Arrangement of DNA |
35 - 36 |
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2.7-2.8 Tree of life and diversity |
37 - 40 |
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4.1-4.2 Cell shape and size |
67 - 70 |
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5.1 Microbial nutrition |
108 - 111 |
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14.1-14.4 Early Earth and life diversification |
368 - 376 |
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14.5-14.9 Microbial evolution |
377 - 385 |
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18.1 Eukaryotic cell |
517 - 518 |
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20.1 Photosynthesis |
579 - 579 |
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Winogradsky and chemolithoautotrophy |
597 - 597 |
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21.1 Fermentation |
613 - 614 |
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21.6 Anaerobic respiration |
624 - 625 |
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22.1 Methods in microbial ecology |
653 - 656 |
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Chap 23 Microbial ecosystems |
673 - 692 |
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24.10 Rumen |
714 - 716 |
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2: Bacterial abundance |
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Porter & Feig (1980) Limnology and Oceanography 25 |
The use of DAPI for identifying and counting aquatic microflora |
943 - 948 |
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Brock Biology of Microorganisms, 12th Ed. |
2.1-2.3 Seeing the very small |
26 - 31 |
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5.3 Lab culture of microorganisms |
113 - 114 |
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6.9-6.10 Cell couting |
153 - 156 |
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22.2-22.3 Isolation and Staining Methods |
657 - 661 |
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36.1 Public health and water qualityh |
1026 - 1028 |
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36.4-36.8 Waterborne diseases |
1033 - 1040 |
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3: Bacterial production |
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Brock Biology of Microorganisms, 12th Ed. |
6.5-6.7 Growth of bacterial populations |
147 - 150 |
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22.7 Measuring microbial activity |
666 - 668 |
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Simon&Azam (1989) Marine Ecology Progress Series 51 |
Protein content and protein synthesis rates of planktonic marine bacteria |
201 - 213 |
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4: Extracellular Enzyme Assays |
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Brock Biology of Microorganisms, 12th Ed. |
3.7-3.8 Proteins and structure |
61 - 64 |
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4.4 Cytoplasmic membranes |
73 - 75 |
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4.7 Outer membrane of Gram-negative bacteria |
82 - 84 |
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5.4-5.5 Energetics and enzymes |
114 - 117 |
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Lehninger (1979) Biochemistry |
Ch 8. Enzymes: kinetics and inhibition |
183 - 195 |
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H.-G. Hoppe (1993) Aquatic microbial ecology |
Ch 48 Use of fluorogenic model substrates for extracellular activity measurements of bacteria |
423 - 431 |
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5: Bacterial Consumers |
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Brock Biology of Microorganisms, 12th Ed. |
18.9 Alveolates |
528 - 530 |
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Caron,D.A., et al. (2012) Annu. Rev. Mar. Sci. 4 |
Marine Protistan Diversity |
467 - 493 |
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Azam et al. (1983) Marine Ecology Progress Series 10 |
The ecological role of water-column microbes in the sea |
257 - 263 |
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6: Chemolithotrophy |
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Brock Biology of Microorganisms, 12th Ed. |
5.6 Oxidation-Reduction |
118 - 119 |
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5.14 Catabolic diversity |
131 - 133 |
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15.2-15.6 Photo-, chemolitho-, and methanotrophs |
400 - 412 |
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15.8 Sulfate- and sulfur reducing proteobacteria |
438 - 441 |
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16.15 Green sulfur bacteria |
474 - 476 |
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16.18 Green nonsulfur bacteria |
481 - 482 |
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17.4 Methanogenes |
494 - 498 |
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20.8-20.13 Chemolithotrophy |
595 - 604 |
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21.7 Nitrate reduction and denitrication |
625 - 627 |
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21.8 Sulfate and sulfur reduction |
627 - 629 |
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21.9 Acetogenesis |
630 - 631 |
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21.10 Methanogenesis |
631 - 635 |
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21.12 Other electron acceptors |
636 - 639 |
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21.16 Methylo- and Methanotrophy |
643 - 644 |
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24.1-24.2 Carbon cycle |
695 - 699 |
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24.3 Nitrogen cycle |
699 - 701 |
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24.4 Sulfur cycle |
701 - 702 |
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24.5 Iron cycle |
703 - 705 |
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7: Microbial food webs: bacteria phytoplankton competition |
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Caron et al. (1988) Hydrobiologia 159 |
Experimental demonstration of the roles of bacteria and bacterivorous protozoa in plankton nutrient cycles. |
27 - 40 |
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8: Molecular techniques |
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Brock Biology of Microorganisms, 12th Ed. |
Chap 13 Microbial Genomics |
343 - 366 |
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22.4-22.6 Molecular methods |
661 - 666 |
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22.8 Stable isotopes |
669 - 671 |
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Head, I.M., J.R. Saunders and R.W. Pickup (1998), Microbial Ecology 35 |
Microbial Evolution, Diversity and Ecology: A Decade of Ribosomal RNA Analysis of Uncultivated microorganisms |
1 - 21 |
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MoBio: Soil DNA isolation kit |
Instruction manual |
1 - 8 |