Reprinted with permission from Johns Hopkins Medical School (2000)

A BACTERIAL DISEASE OF LIMULUS POLYPHEMUS

Frederick Bang


The Marine Biological Laboratories, Woods Hole, Massachusetts, and the Department of Pathobiology,
School of Hygiene and Public Health, The Johns Hopkins University

Received for publication October 12, 1955

Supported in part by a Grant-in-Aid from the National Mogical Institute (E-135) of the U. S. Public Health Service.



FIG. 17. Electron micrograph of serum from normal limulus. This preparation had been freed of hemocyanin by ultracentrifugation. Collodion screens were placed on top of the serum. Toxin was added and the screens removed and the material washed, fixed with osmium vapor. It was then dried and shadowed with chromium.

Similar preparation fifteen minutes after addition of toxin. X 22,000.